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|Type:||Artigo de periódico|
|Title:||Stability and chemical modification of xylanase from Aspergillus sp (2MI strain)|
|Abstract:||The 2M 1 strain of Aspergillus sp,, which showed high extracellular xylanolytic activities in a pre-screening, was studied, Oat-spelt, birch, eucalyptus and pine xylans were used as xylanolytic inductors, The following activities were found at 50 degrees C in the presence of 1% xylan: 120 units/ml (oat-spelt xylan), 132 units/ml (birch xylan), 107 units/ml (eucalyptus :xylan), 67 units/ml (pine xylan) and 137 units/ml (larch-wood xylan), Xylanase induced by pine xylan exhibited a higher stability than those induced by the other xylans, The stability was improved by addition of glycerol, In the crude extract, reagents which were found to affect xylanase activity were 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide for amidation of carboxylic groups and N-bromosuccinimide at a concentration of 0.5 mM for indole oxidation. Methylene Blue, butane-2,3-dione, N-acetylimidazole, chloramine-T and iodoacetate had little effect on the enzyme activity (more than 97% of the original activity remained).|
|Appears in Collections:||Artigos e Materiais de Revistas Científicas - Unicamp|
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