Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/74244
Type: Artigo de periódico
Title: SIMULTANEOUS SPECTROFLUOROMETRIC DETERMINATION OF GLYCEROL AND ETHANOL IN WINE BY FLOW-INJECTION USING IMMOBILIZED ENZYMES
Author: MATTOS, IL
FERNANDEZROMERO, JM
DECASTRO, HDL
VALCARCEL, M
Abstract: An automatic spectrofluorimetric method for the simultaneous determination of glycerol and ethanol is presented. The method is based on the use of a flow injection (FI) manifold and dehydrogenase enzyme immobilized on controlled-pore glass. The proposed method allows for the simultaneous determination of glycerol (linear range between 1 and 10 mu g ml(-1), r = 0.9989 and s(r) <1%) and ethanol (linear range between 10 and 100 mu g ml(-1), r = 0.9998 and s(r) <0.9%). It was applied to the determination of both analytes in different wines with recoveries between 97 and 105%. The sampling frequency was 60 samples h(-1). The results obtained on eight wines were compared with those provided by conventional methods and the agreement between them corroborated the usefulness of the proposed method.
Subject: GLYCEROL AND ETHANOL DETERMINATION
IMMOBILIZED ENZYME
FLOW INJECTION
SPECTROFLUOROMETRY
WINE
Country: Inglaterra
Editor: Royal Soc Chemistry
Rights: aberto
Identifier DOI: 10.1039/an9952000179
Date Issue: 1995
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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