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Type: Artigo de periódico
Title: Synergistic effect of glucose and prolactin on GLUT2 expression in cultured neonatal rat islets
Author: CrepaldiAlves, SC
Carneiro, EM
Bosqueiro, JR
Boschero, AC
Abstract: We studied the synergistic effect of glucose and prolactin (PRL) on insulin secretion and GLUT2 expression in cultured neonatal rat islets. After 7 days in culture, basal insulin secretion (2.3 mM glucose) was similar in control and PRL-treated islets (1.84 +/- 0.06% and 2.08 +/- 0.07% of the islet insulin content, respectively). At 5.6 and 22 mM glucose, insulin secretion was significantly higher in PRL-treated than in control islets, achieving 1.38 +/- 0.15% and 3.09 +/- 0.21% of the islet insulin content in control and 2.43 +/- 0.16% and 4.31 +/- 0.24% of the islet insulin content in PRL-treated islets, respectively. The expression of the glucose transporter GLUT2 in B-cell membranes was dose-dependently increased by exposure of the islet to increasing glucose concentrations. This effect was potentiated in islets cultured for 7 days in the presence of 2 mu g/ml PRL. At 5.6 and 10 mM glucose, the increase in GLUT2 expression in PRL-treated islets was 75% and 150% higher than that registered in the respective control. The data presented here indicate that insulin secretion, induced by different concentrations of glucose, correlates well with the expression of the B-cell-specific glucose transporter GLUT2 in pancreatic islets.
Subject: pancreatic islets
glucose transporter
Country: Brasil
Editor: Assoc Bras Divulg Cientifica
Citation: Brazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 30, n. 3, n. 359, n. 361, 1997.
Rights: aberto
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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