Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/73948
Type: Artigo de periódico
Title: Susceptibility of Streptococcus mutans biofilms to photodynamic therapy: an in vitro study
Author: Zanin, ICJ
Goncalves, RB
Brugnera, A
Hope, CK
Pratten, J
Abstract: Objectives: The purpose of this study was to evaluate the antimicrobial effect of toluidine blue 0 (TBO), in combination with either a helium/neon (HeNe) laser or a light-emitting diode (LED), on the viability and architecture of Streptococcus mutans biofilms. Methods: Biofilms were grown on hydroxyapatite discs in a constant depth film ferment or fed with artificial saliva that was supplemented with 2% sucrose four times a day, thus producing a typical 'Stephan pH curve'. Photodynamic therapy was subsequently carried out on biofilms of various ages with light from either the HeNe laser or LED using energy densities of between 49 and 294 J/cm(2). Results: Significant decreases in the viability of S. mutansbiofilms were only observed when biofilms were exposed to both TBO and light, when reductions in viability of up to 99.99% were observed with both light sources. Overall, the results showed that the bactericidal effect was light dose-dependent and that older biofilms were less susceptible to photodynamic therapy. Confocal laser scanning microscopy images suggested that lethal photosensitization occurred predominantly in the outermost layers of the biofilms. Conclusions: Photodynamic therapy may be a useful approach in the treatment of dental plaque-related diseases.
Subject: dental plaque
caries
Stephan curve
light-emitting diode
biofilm structure
Country: Inglaterra
Editor: Oxford Univ Press
Rights: embargo
Identifier DOI: 10.1093/jac/dki232
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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