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Type: Artigo de periódico
Title: Surface plasmon resonance immunosensor for human cardiac troponin T based on self-assembled monolayer
Author: Dutra, RF
Mendes, RK
da Silva, VL
Kubota, LT
Abstract: The cardiac troponin T (cTnT) is specific biomarker important for trials of acute myocardial infarctions (AMI). In this paper, a SPR sensor in real time to detect the biomarker was developed on a commercially available surface plasmon resonance AUTOLAB SPIRIT(R). The cTnT receptor molecule was covalently immobilized on a gold substrate via a self-assembled monolayer (SAM) of thiols by using cysteamine-coupling chemistry. This biosensor presented a linear response range for cTnT between 0.05 and 4.5 ng/mL (r = 0.997, p << 0.01) with a good reproducibility (CV = 4.4%). The effect of the cysteamine (CYS) concentrations on the SAM coated gold sensor was studied as a function of the amount of the immobilized cTnT monoclonal antibodies. Analysis using serum samples undiluted was carried out at room temperature showing a well agreement with the ECLIA methods and the sensor surface could be regenerated by using a solution of I % (w/v) sodium dodecyl sulphate (SDS) without losing the sensor immunoreactivity. These studies open new perspectives of using SAM to develop regenerable immunosensor with a good reproducibility allowing its use in the clinical applications. (C) 2007 Elsevier B.V. All rights reserved.
Subject: SAM
troponin T
cardiac injury
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.jpba.2006.12.013
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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