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Type: Artigo de periódico
Title: The extract of the jellyfish Phyllorhiza punctata promotes neurotoxic effects
Author: Carneiro, RFV
do Nascimento, NRF
Costa, PPC
Gomes, VM
de Souza, AJF
de Oliveira, SCB
Diz, EBD
Zara, FJ
Fonteles, MC
Toyama, DD
Toyama, MH
Santos, CF
Abstract: Phyllorhiza punctata (P. punctata) is a jellyfish native to the southwestern Pacific. Herewith we present the biochemical and pharmacological characterization of an extract of the tentacles of P. punctata. The tentacles were subjected to three freezethaw cycles, homogenized, ultrafiltered, precipitated, centrifuged and lyophilized to obtain a crude extract (PHY-N). Paralytic shellfish poisoning compounds such as saxitoxin, gonyautoxin-4, tetrodotoxin and brevetoxin-2, as well as several secretory phospholipase A2 were identified. PHY-N was tested on autonomic and somatic neuromuscular preparations. In mouse vas deferens, PHY-N induced phasic contractions that reached a peak of 234 +/- 34.7% of control twitch height, which were blocked with either 100 mu m of phentolamine or 1m m of lidocaine. In mouse corpora cavernosa, PHY-N evoked a relaxation response, which was blocked with either L-NG-Nitroarginine methyl ester (0.5 m m) or 1m m of lidocaine. PHY-N (1, 3 and 10 mu g ml(-1)) induced an increase in tonus of the biventercervicis neuromuscular preparation that was blocked with pre-treatment of galamine (10 mu m). Administration of 6 mg kg(-1) PHY-N intramuscularly produced death in broilers by spastic paralysis. In conclusion, PHY-N induces nerve depolarization and nonspecifically increases neurotransmitter release. Copyright (C) 2011 John Wiley & Sons, Ltd.
Subject: neurotoxicity
marine neurotoxic alkaloids
neuromuscular blockade
smooth muscle spasm
spastic paralysis
nitrergic stimulation
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1002/jat.1620
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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