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Type: Artigo de periódico
Title: The hemibiotrophic cacao pathogen Moniliophthora perniciosa depends on a mitochondrial alternative oxidase for biotrophic development
Author: Thomazella, DPT
Teixeira, PJPL
Oliveira, HC
Saviani, EE
Rincones, J
Toni, IM
Reis, O
Garcia, O
Meinhardt, LW
Salgado, I
Pereira, GAG
Abstract: The tropical pathogen Moniliophthora perniciosa causes witches broom disease in cacao. As a hemibiotrophic fungus, it initially colonizes the living host tissues (biotrophic phase), and later grows over the dead plant (necrotrophic phase). Little is known about the mechanisms that promote these distinct fungal phases or mediate the transition between them. An alternative oxidase gene (Mp-aox) was identified in the M similar to perniciosa genome and its expression was analyzed througout the fungal life cycle. In addition, the effects of inhibitors of the cytochrome-dependent respiratory chain (CRC) and alternative oxidase (AOX) were evaluated on the in vitro development of M similar to perniciosa. Larger numbers of Mp-aox transcripts were observed in the biotrophic hyphae, which accordingly showed elevated sensitivity to AOX inhibitors. More importantly, the inhibition of CRC prevented the transition from the biotrophic to the necrotrophic phase, and the combined use of a CRC and AOX inhibitor completely halted fungal growth. On the basis of these results, a novel mechanism is presented in which AOX plays a role in the biotrophic development of M similar to perniciosa and regulates the transition to its necrotrophic stage. Strikingly, this model correlates well with the infection strategy of animal pathogens, particularly Trypanosoma brucei, which uses AOX as a strategy for pathogenicity.
Subject: alternative oxidase (AOX)
cacao (Theobroma cacao)
Moniliophthora perniciosa
nitric oxide (NO)
phase transition
witches' broom disease (WBD)
Country: EUA
Editor: Wiley-blackwell
Citation: New Phytologist. Wiley-blackwell, v. 194, n. 4, n. 1025, n. 1034, 2012.
Rights: fechado
Identifier DOI: 10.1111/j.1469-8137.2012.04119.x
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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