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Type: Artigo de periódico
Title: Use of chemically modified silica with beta-diketoamine groups for separation of alpha-lactoalbumin from bovine milk whey by affinity chromatography
Author: Gambero, A
Kubota, LT
Gushikem, Y
Airoldi, C
Granjeiro, JM
Taga, EM
Alcantara, EFC
Abstract: Silica gel surface was chemically modified with beta-diketoamine groups by reacting the silanol from the silica surface with 3-aminopropyl-triethoxysilane and 3-bromopentanedione, With this material, copper ions were adsorbed from aqueous solutions, The chemical analysis of the silica-gel-immobilized acetylacetone provided a quantity of 0.67 mmol g(-1) of organic groups attached to the support and 0.63 mmol g(-1) of copper, This material was used as a stationary phase in IMAC (immobilized metal affinity chromatography), to separate alpha-lactoalbumin from bovine milk whey, The results showed an efficient separation in the chromatographic column, The possibility of reutilization of the stationary phase was also investigated. (C) 1997 Academic Press
Subject: modified silica
affinity chromatography
Editor: Academic Press Inc Jnl-comp Subscriptions
Citation: Journal Of Colloid And Interface Science. Academic Press Inc Jnl-comp Subscriptions, v. 185, n. 2, n. 313, n. 316, 1997.
Rights: fechado
Identifier DOI: 10.1006/jcis.1996.4566
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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