Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/73242
Type: Artigo de periódico
Title: Use of a computer controlled hand-scanner for quantitative thin layer chromatographic analysis
Author: Valente, ALP
Augusto, F
Lemes, AC
Lukjanenko, K
Abstract: A microcomputer controlled hand-scanner was used as a densitometer for the quantitation of analytes after separation by TLC (thin layer chromatography). This densitometric process is based on measuring light intensity and counting the number of pixels covering the spots obtained for the analytes after TLC separation. The combination of the aforementioned process with the internal standardization method (ISM) of quantification enabled reliable quantitative data to be obtained for TLC, This methodology, which is simple and relatively inexpensive, has been successfully applied in the TLC analysis of ionic dyes, with less than 2% levels of precision and accuracy.
Country: Inglaterra
Editor: Royal Soc Chemistry
Rights: embargo
Date Issue: 1997
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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