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Type: Artigo de periódico
Title: Validation of a HPLC method for simultaneous determination of main organic acids in fruits and juices
Author: Scherer, R
Rybka, ACP
Ballus, CA
Meinhart, AD
Teixeira, J
Godoy, HT
Abstract: A liquid chromatographic method for fast and simultaneous determination of tartaric, malic, ascorbic and citric acids was validated for further application to fruits and juices. Moreover, the organic acids content of commercial samples of fruits and juices were evaluated, as well as the ascorbic acid stability during the storage. Determination of organic acids was carried out using a liquid chromatograph coupled to a diode array detector, with reversed phase (C-18 column) and isocratic elution with 0.01 mol L-1 KH2PO4 (pH = 2.60) mobile phase. The validation parameters showed efficiency, adequate linearity, relative standard deviation values between 0.4% and 2.3% (n = 10) for repeatability and from 1.2% to 5.0% (n = 18) for reproducibility, limits of detection (LD) were between 0.03 and 3.31 mu g mL(-1) and quantification (LQ) were between 0.10 and 11.03 mu g mL(-1), recovery rates were between 82% and 110%, for two levels. In addition, the method is fast (10 min) and generates low and non-toxic residues. The values found for vitamin C were about 10 times above the values declared at the package. Ready to drink juices have a composition similar to the fruit, concerning to organic acids, except for the powder juice, in which only ascorbic and citric acids were found, for all tastes. After opening the package, a decrease of 14.0% and 27.0% in ascorbic acid content was observed for orange powder and ready to drink juices, respectively. (c) 2012 Elsevier Ltd. All rights reserved.
Subject: Liquid chromatography
Organic acids
Method validation
Fruit juices
Ascorbic acid stability
Country: Inglaterra
Editor: Elsevier Sci Ltd
Citation: Food Chemistry. Elsevier Sci Ltd, v. 135, n. 1, n. 150, n. 154, 2012.
Rights: fechado
Identifier DOI: 10.1016/j.foodchem.2012.03.111
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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