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Type: Artigo de periódico
Title: Search for herpesvirus 1 and 2 by in situ hybridization in tonsils and adenoids
Author: Vassallo, J
de Camargo, LA
Chagas, CA
Pinto, GA
Endo, LH
Abstract: Objective: Herpes simplex virus (HSV) has been described as cause of acute tonsillitis. It has also been found in nasopharyngeal florid lymphoid infiltrate, mostly composed of CD4+, CD56+ T-cells, simulating lymphoma. In spite of its widespread prevalence in latent form, to the best of our knowledge no study is available on in situ detect-ion of HSV in chronically hyperplastic nasopharyngeal lymphoid tissue. The purpose of the present study was to search for the presence of HSV 1 and 2 in 21 adenoids and 15 tonsils from children (2-12 years of age) in which these organs had been surgically removed due to hypertrophy. Methods: Paraffin wax-embedded sections from the 36 cases were submitted to the in situ hybridization technique, using the biotinilated probe to Herpes simplex virus 1 and 2 (Pan Path, Amsterdam) and the Rembrandt (R) Universal DISH M HRP Detection Kit (Pan Path, Amsterdam). Positive control consisted of a previously tested Herpes infected lung. Results: In none of the 36 cases studied were positive nuclei detected in adenoid and tonsils, either in lymphoid, in stroma or in epithelial, cells, as those seen in the positive control. Conclusion: HSV does not seem to be implied in tonsil. or adenoid chronic lymphoid hyperplasia. These organs do not seem to harbor the virus latently, or the amount of virus is too low to be detected without amplification methods. (c) 2004 Elsevier Ireland Ltd. All rights reserved.
Subject: herpes simplex virus 1 and 2
in situ hybridization
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: International Journal Of Pediatric Otorhinolaryngology. Elsevier Sci Ireland Ltd, v. 69, n. 3, n. 345, n. 349, 2005.
Rights: fechado
Identifier DOI: 10.1016/j.ijporl.2004.10.006
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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