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Type: Artigo de periódico
Title: Screening of reducing agents for anaerobic growth of Candida albicans SC5314
Author: Rymovicz, AUM
Souza, RD
Gursky, LC
Rosa, RT
Trevilatto, PC
Groppo, FC
Rosa, EAR
Abstract: This study aimed to evaluate the influence of different redox potentials (Eh) on cell growth, whole-cell protein profile and cell surface hydrophobicity (CSH) of Candida albicans SC5314. The yeast was grown in YNB broth enriched with reducing (158 mM sodium sulfite, 4 mM sodium sulfite, 2.5 mM sodium metabisulfite, 13 mM 2-mercaptoethanol, 5.5 mM thioglycolic acid, and 32 mM L-cysteine hydrochloride) and oxidizing agents (15 mM ammonium persulfate and 80 mM potassium ferricyanide) and incubated in normoxic and anoxic atmospheres at 37 degrees C, for 48 h. Pre- and post-incubation Eh values were determined and cytoplasm proteins were extracted. Proteins were parted by SDS-PAGE and their profiles were compared. 32 mM L-cysteine and 13 mM 2-mercaptoethanol promoted and maintained negative Eh values during incubation. No differences were detected among SDS-PAGE profiles. CSH differences only were observed with 4 mM sodium sulfite and 3.2 mM L-cysteine. Results showed that 32 mM L-cysteine is a reducing agent that allows maintenance of negative Eh in both anoxic and normoxic conditions and it seems not to interfere in the global expression of plasmatic proteins. (C) 2011 Elsevier B.V. All rights reserved.
Subject: Candida albicans
Reducing agents
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.mimet.2011.01.020
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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