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Type: Artigo
Title: Screening of reducing agents for anaerobic growth of Candida albicans SC5314
Author: Rymovicz, A. U. M.
Souza, R. D.
Gursky, L. C.
Rosa, R. T.
Trevilatto, P. C.
Groppo, F. C.
Rosa, E. A. R.
Abstract: This study aimed to evaluate the influence of different redox potentials (Eh) on cell growth, whole-cell protein profile and cell surface hydrophobicity (CSH) of Candida albicans SC5314. The yeast was grown in YNB broth enriched with reducing (158 mM sodium sulfite, 4 mM sodium sulfite, 2.5 mM sodium metabisulfite, 13 mM 2-mercaptoethanol, 5.5 mM thioglycolic acid, and 32 mM L-cysteine hydrochloride) and oxidizing agents (15 mM ammonium persulfate and 80 mM potassium ferricyanide) and incubated in normoxic and anoxic atmospheres at 37 degrees C, for 48 h. Pre- and post-incubation Eh values were determined and cytoplasm proteins were extracted. Proteins were parted by SDS-PAGE and their profiles were compared. 32 mM L-cysteine and 13 mM 2-mercaptoethanol promoted and maintained negative Eh values during incubation. No differences were detected among SDS-PAGE profiles. CSH differences only were observed with 4 mM sodium sulfite and 3.2 mM L-cysteine. Results showed that 32 mM L-cysteine is a reducing agent that allows maintenance of negative Eh in both anoxic and normoxic conditions and it seems not to interfere in the global expression of plasmatic proteins
Subject: Candida albicans
Country: Holanda
Editor: Elsevier
Citation: Journal Of Microbiological Methods. Elsevier Science Bv, v. 84, n. 3, n. 461, n. 466, 2011.
Rights: fechado
Identifier DOI: 10.1016/j.mimet.2011.01.020
Date Issue: 2011
Appears in Collections:FOP - Artigos e Outros Documentos

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