Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/71560
Type: Artigo de periódico
Title: SCREENING OF MICROBIAL LIPASES AND EVALUATION OF THEIR POTENTIAL TO PRODUCE GLYCERIDES WITH HIGH GAMMA LINOLENIC ACID CONCENTRATION
Author: Fregolente, PBL
Fregolente, LV
Maciel, MRW
Carvalho, PO
Abstract: Gamma-linolenic acid (GLA, 18: 3, cis-6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special focus on enrichment of GLA were studied and compared with those obtained by two commercially-available lipases. Only one of the lipases tested during this study displayed selectivity, discriminating the GLA during the hydrolysis reaction. Using the enzymatic extract from Geotrichum candidum as a biocatalyst of the reaction, it was possible to obtain a percentage of 41.7% of GLA in acylglycerols fraction when the borage oil was treated in a fixed-bed reactor for 24 hours at 30 degrees C.
Subject: gamma linolenic acid
borage oil
lipase
Geotrichum candidum
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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