Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/71458
Type: Artigo de periódico
Title: Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa
Author: Nogueira, RT
Queiroz, SCN
Imamura, PM
Abstract: Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol: water: formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).
Country: EUA
Editor: Marcel Dekker Inc
Rights: fechado
Identifier DOI: 10.1081/JLC-100108539
Date Issue: 2002
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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