Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/71289
Type: Artigo de periódico
Title: Secretome of the preimplantation human embryo by bottom-up label-free proteomics
Author: Cortezzi, SS
Garcia, JS
Ferreira, CR
Braga, DPAF
Figueira, RCS
Iaconelli, A
Souza, GHMF
Borges, E
Eberlin, MN
Abstract: A bottom-up label-free mass spectrometric proteomic strategy was used to analyse the protein profiles of the human embryonic secretome. Culture media samples used for embryonic culture of patients undergoing intracytoplasmic sperm injection cycles were selected as a test case for this exploratory proof-of-principle study. The media were stored after embryo transfer and then pooled into positive (n = 8) and negative (n = 8) implantation groups. The absolute quantitative bottom-up technique employed a multidimensional protein identification technology based on separation by nano-ultra-high pressure chromatography and identification via tandem nano-electrospray ionization mass spectrometry with data-independent scanning in a hydrid QqTOF mass spectrometer. By applying quantitative bottom-up proteomics, unique proteins were found exclusively in both the positive- and negative-implantation groups, which suggest that competent embryos express and secrete unique biomarker proteins into the surrounding culture medium. The selective monitoring of these possible secretome biomarkers could make viable procedures using single-embryo transfer.
Subject: Bioanalytical methods
Mass spectrometry
Proteomics
Label-free quantitation
In vitro fertilization
Embryo secretome
Country: Alemanha
Editor: Springer Heidelberg
Rights: fechado
Identifier DOI: 10.1007/s00216-011-5202-1
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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