Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/71237
Type: Artigo de periódico
Title: Searching for monooxygenases and hydrolases in bacteria from an extreme environment
Author: da Cruz, GF
Angolini, CFF
de Oliveira, LG
Lopes, PF
de Vasconcellos, SP
Crespim, E
de Oliveira, VM
Neto, EVD
Marsaioli, AJ
Abstract: Microbial oxidation potentials of extremophiles recovered from Pampo Sul oil field, Campos Basin, Brazil, in pure culture or in consortia, were investigated using high-throughput screening (HTS) and multibioreactions. Camphor (1), cis-jasmone (2), 2-methyl-cyclohexanone (3), 1,2-epoxyoctane (4), phenylethyl acetate (5), phenylethyl propionate (6), and phenylethyl octanoate (7) were used to perform multibioreaction assays. Eighty-two bacterial isolates were recovered from oil and formation water samples and those presenting outstanding activities in HTS assays were identified by sequencing their 16S rRNA genes. These results revealed that most microorganisms belonged to the genus Bacillus and presented alcohol dehydrogenase, monooxygenase, epoxide hydrolase, esterase, and lipase activities.
Subject: Oil reservoirs
Alcohol dehydrogenases
Monooxygenases
Hydrolases
High-throughput screening
Bacterial 16S rRNA genes
Petroleum microorganisms
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s00253-010-2485-7
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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