Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/71092
Type: Artigo de periódico
Title: Retinyl palmitate polymeric nanocapsules as carriers of bioactives
Author: Teixeira, Z
Dreiss, CA
Lawrence, MJ
Heenan, RK
Machado, D
Justo, GZ
Guterres, SS
Duran, N
Abstract: Nanocapsules containing poly(D,L-lactide) shell and retinyl palmitate core have been prepared by the preformed polymer interfacial deposition method. Dynamic light scattering measurements yielded an average hydrodynamic diameter of similar to 220 nm and a polydispersity index of similar to 0.12. Small-angle neutron scattering experiments revealed the presence of two populations of nanocapsules of core diameters similar to 192 and 65 nm. Freeze fracture transmission electron microscopy showed a polydisperse population of nanocapsules (NC), with a poly(D,L-lactide) shell thickness between 11 and 3 nm. For comparison purposes, nanoemulsions (NE, no polymer) and nanospheres (NS, polymer matrix) were also prepared. Each type of nanoparticles exhibited a different morphology (when examined by electron microscopy), in particular NC showed deformability by capillary adhesion. All three types of nanoparticles successfully encapsulated the poorly water-soluble molecules baicalein and benzophenone-3. The thermal behavior of the various nanoparticles was different to a physical mixture of its individual components. Cytotoxicity and phototoxicity assays, performed in human keratinocytes (HaCaT) and murine fibroblasts (BALB/c3T3), showed that the NC were only cytotoxic at high concentrations. In vitro release studies of benzophenone-3, by the dialysis bag method using NC and NS, showed a sustained release; however, permeation studies using plastic surgery human abdominal skin in Franz diffusion cells showed that a higher amount of benzophenone-3 from NC penetrated into the skin, most probably due to the deformable nature of these nanoparticles. (C) 2012 Elsevier Inc. All rights reserved.
Subject: Polymeric nanoparticles
Poly(D,L-lactide)
Nanocapsules
Skin permeation
Phototoxicity
Cytotoxicity
Small-angle neutron scattering
Country: EUA
Editor: Academic Press Inc Elsevier Science
Rights: fechado
Identifier DOI: 10.1016/j.jcis.2012.05.042
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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