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Type: Artigo de periódico
Title: Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5
Author: D'Arce, RCF
Almeida, RS
Silva, TC
Franco, AC
Spilki, F
Roehe, PM
Arns, CW
Abstract: Twelve Brazilian isolates and three reference strains of bovine herpesviruses (BHVs) were subjected to restriction endonuclease analysis (REA) and monoclonal antibody (MAb) analysis. Viral DNA was cleaved with BamHI, BstEII, EcoRI, HindIII and PstI. The monoclonal antibody panel allowed the differentiation between types I and 5 viruses, while REA with BstEII and HindIII showed the distinction between BHV-1 and -5 subtypes. Typical 1.1 and 1.2a patterns were observed with two isolates from respiratory disease. An isolate from semen of a clinically healthy bull displayed 1.2b, profile, whereas another displayed a clear 5a pattern, which was never reported before. Seven out of nine Brazilian type 5 (BHV-5) isolates displayed REA patterns similar to the Australian BHV-5 strain N569 (BHV-5a), and differing from the Argentinean A663 strain (BHV-5b) virus. Another two BHV-5 isolates, which displayed an unusual MAb pattern of reactivity, showed a BstEII profile different from both reference strains of BHV-5. These two viruses were considered BHV-5 "non-a/non-b" subtype. (C) 2002 Elsevier Science B.V. All rights reserved.
Subject: bovine herpesvirus type 1 (BHV-1)
bovine herpesvirus type 5 (BHV-5)
restriction endonuclease analysis
monoclonal antibodies
Country: Holanda
Editor: Elsevier Science Bv
Citation: Veterinary Microbiology. Elsevier Science Bv, v. 88, n. 4, n. 315, n. 324, 2002.
Rights: fechado
Identifier DOI: 10.1016/S0378-1135(02)00126-8
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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