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Type: Artigo de periódico
Title: Response surface analysis for the production of an enantioselective lipase from Aspergillus niger by solid-state fermentation
Author: Contesini, FJ
da Silva, VCF
Maciel, RF
de Lima, RJ
Barros, FFC
Carvalho, PD
Abstract: The lipase produced by the Aspergillus niger strain AC-54 has been widely studied due to its enantioselectivity for racemic mixtures. This study aimed to optimize the production of this enzyme using statistical methodology. Initially a Plackett-Burman (PB) design was used to evaluate the effects of the culture medium components and the culture conditions. Twelve factors were screened: water content, glucose, yeast extract, peptone, olive oil, temperature, NaH(2)P0(4), KH(2)P0(4), MgS0(4)-7H(2)0, CaCl(2), NaCI, and MnS0(4). The screening showed that the significant factors were water content, glucose, yeast extract, peptone, NaH(2)P0(4), and KH(2)P0(4), which were optimized using response surface methodology (RSM) and a mathematical model obtained to explain the behavioral process. The best lipase activity was attained using the following conditions: water content (20%), glucose (4.8%), yeast extract (4.0%), and NaH2P04 (4.0%). The predicted lipase activity was 33.03 U/ml and the experimental data confirmed the validity of the model. The enzymatic activity was expressed as A mu moles of oleic acid released per minute of reaction (A mu mol/min).
Subject: lipase
response surface methodology
A. niger
Country: Coreia do Sul
Editor: Microbiological Society Korea
Citation: Journal Of Microbiology. Microbiological Society Korea, v. 47, n. 5, n. 563, n. 571, 2009.
Rights: fechado
Identifier DOI: 10.1007/s12275-008-0279-8
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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