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Type: Artigo de periódico
Title: Respiratory chain network in mitochondria of Candida parapsilosis: ADP/O appraisal of the multiple electron pathways
Author: Milani, G
Jarmuszkiewicz, W
Sluse-Goffart, CM
Schreiber, AZ
Vercesi, AE
Sluse, FE
Abstract: In this study we demonstrated that mitochondria. of Candida parapsilosis contain a constitutive ubiquinol alternative oxidase (AOX) in addition to a classical respiratory chain (CRC) and a parallel respiratory chain (PAR) both terminating by two different cytochrome c oxidases. The C. parapsilosis AOX is characterized by a fungi-type regulation by GMP (as a stimulator) and linoleic acid (as an inhibitor). Inhibitor screening of the respiratory network by the ADP/O ratio and state 3 respiration determinations showed that (i) oxygen can be reduced by the three terminal oxidases through four paths implying one bypass between CRC and PAR and (ii) the sum of CRC, AOX and PAR capacities is higher than the overall respiration (no additivity) and that their engagement could be progressive according to the redox state of ubiquinone, i.e. first cytochrome pathway, then AOX and finally PAR. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Subject: mitochondrion
electron transport route
alternative oxidase
Candida parapsilosis
Country: Holanda
Editor: Elsevier Science Bv
Citation: Febs Letters. Elsevier Science Bv, v. 508, n. 2, n. 231, n. 235, 2001.
Rights: fechado
Identifier DOI: 10.1016/S0014-5793(01)03060-5
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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