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Type: Artigo de periódico
Title: Polyphenoloxidase activity in coffee leaves and its role in resistance against the coffee leaf miner and coffee leaf rust
Author: Melo, GA
Shimizu, MM
Mazzafera, P
Abstract: In plants.. PPO has been related to defense mechanism against pathogens and insects and this role was investigated in coffee trees regarding resistance against a leaf miner and coffee leaf rust disease. PPO activity was evaluated in different genotypes and in relation to methyl-jasmonate (Meja) treatment and mechanical damage. Evaluations were also performed using compatible and incompatible interactions of coffee with the fungus Hemileia vastatrix (causal agent of the leaf orange rust disease) and the insect Leucoptera coffeella (coffee leaf miner). The constitutive level of PPO activity observed for the 15 genotypes ranged from 3.8 to 88 units of activity/mg protein. However, no direct relationship was found with resistance of coffee to the fungus or insect. Chlorogenic acid (5-caffeoylquinic acid), the best substrate for coffee leaf PPO, was not related to resistance, suggesting that oxidation of other phenolics by PPO might play a role, as indicated by HPLC profiles. Mechanical damage, Meja treatment, H. vastatrix fungus inoculation and L. coffeella infestation caused different responses in PPO activity. These results suggest that coffee resistance may be related to the oxidative potential of the tissue regarding the phenolic composition rather than simply to a higher PPO activity. (c) 2005 Elsevier Ltd. All rights reserved.
Subject: Coffea
Hemilea vastatrix
Leucoptera coffeela
phenolic compounds
plant resistance
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Citation: Phytochemistry. Pergamon-elsevier Science Ltd, v. 67, n. 3, n. 277, n. 285, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.phytochem.2005.11.003
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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