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Type: Artigo de periódico
Title: Polymer-based alternative method to extract bromelain from pineapple peel waste
Author: Novaes, LCD
Ebinuma, VDS
Mazzola, PG
Pessoa, A
Abstract: Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol (PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process. (C) 2013 International Union of Biochemistry and Molecular Biology, Inc. Volume 60, Number 5, Pages 527-535, 2013
Subject: aqueous two-phase system
pineapple waste
polyethylene glycol
poly(acrylic acid)
Country: EUA
Editor: Wiley-blackwell
Citation: Biotechnology And Applied Biochemistry. Wiley-blackwell, v. 60, n. 5, n. 527, n. 535, 2013.
Rights: fechado
Identifier DOI: 10.1002/bab.1121
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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