Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/70689
Type: Artigo de periódico
Title: PolyHEMA and polyHEMA-poly(MMA-co-AA) as substrates for culturing Vero cells
Author: Lombello, CB
Malmonge, SM
Wada, MLF
Abstract: Poly (2-hydroxyethyl methacrylate), polyHEMA, is known to prevent cellular attachment and spreading. This hydrogel is used to culture cells not dependent on anchorage. Blending polyHEMA with a copolymer of methyl methacrylate and acrylic acid introduces negative charges to the hydrogel and improves its mechanical characteristics. PolyHEMA and the blend were tested for attachment and proliferation of Vero cells. Dense and porous samples of the hydrogels were used. Attachment assays included cellular quantification with MTT photometry and cellular morphology with the scanning electron microscopy after 2 h culture. Proliferation assays were carried out with 5 and 10 days culture. Cellular morphology included cytochemistry of resin sections and scanning electron microscope observations. Hydrogels allowed a few cells to attach and proliferate. The cells growing on the surface of hydrogels were organized in various layers and showed a differential morphology. Cells located inside the pores remained rounded. The hydrogels showed the possibility of inducing differentiated phenotypic expression. (C) 2000 Kluwer Academic Publishers.
Country: Holanda
Editor: Kluwer Academic Publ
Rights: fechado
Identifier DOI: 10.1023/A:1008915801187
Date Issue: 2000
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File Description SizeFormat 
WOS000088981200002.pdf242.92 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.