Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/70680
Type: Artigo de periódico
Title: Polyester-toner electrophoresis microchips with improved analytical performance and extended lifetime
Author: Gabriel, EFM
Duarte, GF
Garcia, PD
de Jesus, DP
Coltro, WKT
Abstract: This paper reports the fabrication of polyester-toner (PT) electrophoresis microchips with improved analytical performance and extended lifetime. This has been achieved with a better understanding about the EOF generation and the influence of some parameters including the channel dimensions (width and depth), the injection mode, and the addition of organic solvent to the running buffer. The analytical performance of the PT devices was investigated using a capacitively coupled contactless conductivity detector and inorganic cations as model analytes. The proposed devices have exhibited EOF values of (3.4 +/- 0.2) x 10-4 cm2 V-1 s-1 with good stability over 25 consecutive runs. It has been found that the EOF magnitude depends on the channel dimension, i.e. the wider the channel, the higher the EOF value. The separation efficiency for inorganic cations ranged from 13 000 to 50 000 plates/m. The LOD found for K+, Na+, and Li+ were 4.2, 7.3, and 23 mu M, respectively. In addition, the same PT device has been used by three consecutive days. Lately, due to improved analytical performance, it was carried out by the first time the detection of inorganic cations in real samples such as energetic drinks and pharmaceutical formulations.
Subject: Contactless conductivity detection
Direct-printing process
Electrophoresis microchips
Substrate materials
Country: EUA
Editor: Wiley-blackwell
Rights: fechado
Identifier DOI: 10.1002/elps.201200009
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000308581100006.pdf673.35 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.