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Type: Artigo de periódico
Title: PmTCP1 encodes a putative TCP transcription factor and is differentially expressed during in vitro organogenesis in Passiflora
Author: Rosa, YBCJ
Aizza, LCB
Bello, CCM
Dornelas, MC
Abstract: The genus Passiflora includes economically important passion fruits and over 600 other wild species. Micropropagation of Passiflora species is far from routine due to low regeneration frequencies and multiplication rates, so there is great interest in understanding the molecular control of the in vitro regeneration processes of these species. Here, we report the characterization of a Passiflora morifolia gene encoding PmTCP1, a putative TCP transcription factor that showed high sequence similarity to Arabidopsis class I TCPs. The expression patterns of the PmTCP1 gene during in vitro organogenesis and callus growth revealed differential expression of PmTCP1 modulated by different combinations of auxin and cytokinin concentrations in the culture medium. At a constant auxin concentration, cytokinin increased PmTCP1 expression, and at a constant cytokinin concentration, auxin repressed PmTCP1 expression. We also observed a correlation between PmTCP1 expression and the in vitro organogenesis of roots and shoots. We expect that these results will increase our understanding of the molecular networks and environmental signals that modulate the processes of organogenesis during plant development.
Subject: Auxin
Wild passion fruit
In vitro culture
Differential gene expression
Country: EUA
Editor: Springer
Citation: In Vitro Cellular & Developmental Biology-plant. Springer, v. 50, n. 1, n. 36, n. 44, 2014.
Rights: fechado
Identifier DOI: 10.1007/s11627-013-9585-x
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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