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Type: Artigo de periódico
Title: Production, extraction and purification of violacein: an antibiotic pigment produced by Chromobacterium violaceum
Author: Rettori, D
Duran, N
Abstract: A procedure for the production, extraction, and purification of violacein was developed using Chromobacterium violaceum (CCT 3496) cultivated on cotton, in modified 1 litre Roux bottles. A surface tray bioreactor was built to perform these experiments. Violacein was extracted with commercial ethanol, and purified by filtration, Soxhlet extraction, crystallization and high performance liquid chromatography. The violacein was analysed and identified by proton and carbon-13 NMR spectroscopies, thermogravimetric analysis, mass spectrometry, UV-VIS spectroscopy and infrared spectroscopy. It was concluded that the product was highly purified violacein.
Subject: antibiotic
Chromobacterium violaceum
Country: Holanda
Editor: Kluwer Academic Publ
Citation: World Journal Of Microbiology & Biotechnology. Kluwer Academic Publ, v. 14, n. 5, n. 685, n. 688, 1998.
Rights: fechado
Identifier DOI: 10.1023/A:1008809504504
Date Issue: 1998
Appears in Collections:Unicamp - Artigos e Outros Documentos

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