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Type: Artigo de periódico
Title: Production of isomaltulose using Erwinia sp D12 cells: Culture medium optimization and cell immobilization in alginate
Author: Kawaguti, HY
Manrich, E
Sato, HH
Abstract: Isomaltulose is a structural isomer of sucrose commercially used in food industries. Glucosyltransferase produced by Erwinia sp. D12 catalyses an intramolecular transglucosylation of sucrose giving isomaltulose. An experimental Design and Response Surface Methodology were applied for the optimization of the nutrient concentration in the culture medium for enzyme production in shaken flasks at 200 rpm and 30 degrees C. A higher production of glucosyltransferase (7.47 Uml(-1)) was observed in the culture medium containing sugar cane molasses (160 gl(-1)), bacteriological peptone (20 gl(-1)) and yeast extract Prodex Lac SD (R) (15 gl(-1)) after 8 h, at 30 degrees C. The highest production of glucosyltransferase in the 6.6-1 bioreactor (14.6 Uml(-1)) was obtained in the optimized culture medium after 10 h at 26 degrees C. When Erwinia sp. D12 cells were immobilized in sodium alginate, it was verified that sodium alginate solution A could be substituted by a cheaper one, sodium alginate solution B. Using a 40% cell suspension and 2% sodium alginate solution B for cell immobilization in a packed-bed reactor, 64.1% conversion of sucrose to isomaltulose was obtained. The packed-bed reactor with immobilized cells plus glutaraldehyde and polyethylenimine solutions remained in a pseudo-steady-state for 180 h. (c) 2006 Elsevier B.V. All rights reserved.
Subject: experimental designs
immobilized cells
packed-bed reactor
response surface methodology
Country: Suíça
Editor: Elsevier Science Sa
Rights: fechado
Identifier DOI: 10.1016/j.bej.2006.01.006
Date Issue: 2006
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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