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Type: Artigo de periódico
Title: Production of glucosyltransferase by Erwinia sp using experimental design and response surface methodology
Author: Kawaguti, HY
Manrich, E
Fleuri, LF
Sato, HH
Abstract: Glucosyltransferase produced by strain Erwinia sp. is an intracellular enzyme that catalyzes the formation of isomaltulose from sucrose. Isomaltulose is a non-cariogenic reducing dissacharide commercially used in foods. Response surface methodology and 2(3)-factorial central composite design were employed to optimize a fermentation medium for the production of glucosyltransferase by Erwinia sp. in shaken flasks at 200 rpm and 30 degrees C. The three variables involved in this study were sugar cane molasses (SCM), corn steep liquor (CSL) and yeast extract Prodex Lac SD (YEP). The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect on glucosyltransferase production and the optimum medium composition for enzyme production was (in g l(-1)) SCM-100, CSL-60 and YEP-8, which lead to a glucosyltransferase activity of 6.65 U mL(-1).
Subject: glucosyltransferase
response surface
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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