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|Type:||Artigo de periódico|
|Title:||Production of branched cyclodextrins by reverse reaction of microbial debranching enzymes|
|Abstract:||Formation of maltosyl cyclodextrins from mixtures of maltose and cyclodextrins by reverse reactions of Flavobacterium isoamylase and Klebsiella pullulanase was experimented and it was found that Klebsilla pullulanase produced 50.4 mg maltosyl alpha-cyclodextrin, 35.0 mg maltosyl beta-cyclodextrin and 55.4 mg maltosyl gamma-cyclodextrin per ml of reaction mixture, whereas Flavobacterium isoamylase did not form maltosyl cyclodextrins. Optimum conditions for formation of maltosyl beta-cyclodextrin by Klebsiella pullulanase were pH 4, 50 degrees C reaction temperature and proportion of substrate 100 mg beta-cyclodextrin/600 mg maltose per ml.|
|Editor:||Vch Publishers Inc|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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