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Type: Artigo de periódico
Title: Production of an acidic and thermostable lipase of the mesophilic fungus Penicillium simplicissimum by solid-state fermentation
Author: Gutarra, MLE
Godoy, MG
Maugeri, F
Rodrigues, MI
Freire, DMG
Castilho, LR
Abstract: The production of a lipase by a wild-type Brazilian strain of Penicillium simplicissimium in solid-state fermentation of babassu cake, an abundant residue of the oil industry, was studied. The enzyme production reached about 90 U/g in 72 h, with a specific activity of 4.5 U/mg of total proteins. The Crude lipase showed high activities at 35-60 degrees C and pH 4.0-6.0, with a maximum activity at 50 degrees C and pH 4.0-5.0. Enzyme stability was enhanced at pH 5.0 and 6.0, with a maximum half-life of 5.02 h at 50 degrees C and pH 5.0. Thus, this lipase shows a thermophilic and thermostable behavior, what is not common among lipases from mesophilic filamentous fungi. The crude enzyme catalysed the hydrolysis of triglycerides and p-nitrophenyl esters (C4:0-C18:0), preferably acting on substrates with medium-chain fatty acids. This non-purified lipase in addition to interesting properties showed a reduced production cost making feasible its applicability in many fields. (C) 2009 Elsevier Ltd. All rights reserved.
Subject: Solid-state fermentation
Babassu cake
Penicillium simplicissimum
Acidic lipase
Thermal stability
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.biortech.2008.08.050
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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