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Type: Artigo de periódico
Title: Prevalence of virulence factors in Escherichia coil isolated from healthy animals and water sources in Brazil
Author: Carlos, C
Alexandrino, F
Vieira, MAM
Stoppe, NC
Sato, MIZ
Gomes, TAT
Ottoboni, LMM
Abstract: The aim of this work was to verify the presence of seven virulence factors (ST. LT, eae, stx(1), stx(2), INV and EAEC) among Escherichia coli strains isolated from healthy humans, bovines, chickens, sheep, pigs and goats, from two sewage treatment plants and from the Tiete River. We have found a high prevalence of eae, stx(1) and stx(2) in ruminants. The EAEC gene was only found in humans and sewage. No strains presented ST, LT or INV. BOX-PCR fingerprints revealed a high diversity among the strains analysed and a non-clonal origin of strains that presented the same virulence factors. Therefore, we concluded that ruminants may constitute an important reservoir of most diarrheagenic E. coli in Brazil, except for EAEC strains. These results emphasize the importance of the identification of the animal source of fecal contamination for the correct water risk assessment.
Subject: BOX-PCR
Escherichia coli
fecal contamination
virulence factors
Country: Inglaterra
Editor: Iwa Publishing
Rights: fechado
Identifier DOI: 10.2166/wh.2010.068
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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