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Type: Artigo de periódico
Title: Preparation and characterization of diacetylene polymerized liposomes for detection of autoantibodies
Author: Cabral, ECM
Hennies, PT
Correia, CRD
Zollner, RL
Santana, MHA
Abstract: Affinity diacetylene liposomes were prepared with 10,12-tricosadiynoic acid and cardiolipin as the affinity ligand for anticardiolipin antibodies at a molar ratio of 80:20. Polymerization was carried out under UV irradiation, and the color transitions were monitored by visible absorption spectroscopy. Peaks at 63 5 nm (blue form), 540 nm (purple form), and 480 nm (red form) were observed as a function of time. These polymerized liposomes were used in a noncompetitive immunoassay for detection of anticardiolipin antibodies. Color changes were observed when reference serum containing specific immunoglobulin G, IgG, was added to polymerized liposome dispersions. The colorimetric signal due to IgG adsorption on the liposome surface was quantified as a colorimetric response defined as the change in percentage of blue color related to the initial percentage of blue color in the solutions. The colorimetric response was 10 times higher for specific IgG compared with nonspecific ones. These results suggest the unique potentialities of affinity diacetylene polymerized liposomes in the development of biosensors for diagnosis of autoimmune diseases.
Subject: polymerized liposomes
anti-cardiolipin antibodies
Country: EUA
Editor: Marcel Dekker Inc
Citation: Journal Of Liposome Research. Marcel Dekker Inc, v. 13, n. 41732, n. 199, n. 211, 2003.
Rights: fechado
Identifier DOI: 10.1081/LPR-120026387
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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