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Type: Artigo de periódico
Title: Immunological effects of interferon-alpha on chronic myelogenous leukemia
Author: de Castro, FA
Palma, PVB
Morais, FR
Simoes, BP
Carvalho, PVB
Ismael, SJ
Lima, CP
Voltarelli, JC
Abstract: Treatment with interferon-alpha is effective for chronic myelogenous leukemia in the chronic phase (CML-CP), but the immunological mechanisms of the antileukemic effect of this substance are still unclear. The objective of this study was to investigate the immunological effects of interferon-alpha in CML patients. Markers of cellular activation and apoptosis, natural killer (NK) cell cytotoxicity and production of intracellular cytokines (IFN-gamma, IL-2 and IL-4) were determined by flow cytometry in the peripheral blood mononuclear cells (PBMC) of 26 CML-CP patients before and 3, 6 and 9 months after IFN-alpha treatment. The results were correlated with the hematological response. In the whole group of patients, INF-alpha use was followed by a significant increase of lymphocytes producing IL-2 and IFN-gamma, an increase in NK activity and a decrease in the number of CD34 + cells. Out of 26 CML patients, 15 achieved hematological remission and 7 achieved partial cytogenetic remission after 9 months of IFN-alpha treatment. There was an increase in the percentage of CD8/FasL + , DR/CD3 + , DQ/CD3 + , CD34/Fas + , DR/CD56 + , CD56/FasL + cells and of IFN-gamma- and IL-2-producing lymphocytes and an increase in NK cytotoxicity only in the group of patients who achieved complete hematological remission. Our results indicate that IFN-alpha use in CML-CP reduces the number of CD34 + cells, activates T cells, enhances stem cell apoptotic markers and increases the production of intracellular IFN-gamma and IL-2 by lymphocytes. Taken together, these results indicate that the therapeutic effect of IFN-alpha in CML-CP is mediated at least in part by immunological mechanisms.
Subject: interferon-alpha
chronic myelogenous leukemia
cytokines and NK cytotoxicity
Country: Inglaterra
Editor: Taylor & Francis Ltd
Citation: Leukemia & Lymphoma. Taylor & Francis Ltd, v. 44, n. 12, n. 2061, n. 2067, 2003.
Rights: fechado
Identifier DOI: 10.1080/1042819031000110973
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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