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Type: Artigo de periódico
Title: Immunohistochemical staining of the macrophagic and astrocytic response in the brainstern of Wistar rats submitted to the ethidium bromide gliotoxic model and treated with cyclophosphamide
Author: Sanchez, M
Bondan, EF
Lallo, MA
Sinhorini, IL
Dagli, MLZ
Maiorka, PC
Pereira, LAVD
Graca, DL
Abstract: The gliotoxic ethidium bromide (EB) was used to study morphologically the macrophagic and astrocytic response under immunosuppression by cyclophosphamide (CY). Astrocyte immunoreactivity to glial fibrillary acidic protein (GFAP) and vimentin (VIM) and macrophagic immunoreactivity to ED1 were investigated after EB injection. Male Wistar rats were injected with 0.9% saline solution (group 1), 0.1% BE (group II) and 0.1% EB associated with CY treatment (group III). Brainstem samples were collected from the 1(st) to the 21(st) day post-injection for GFAP, VIM and ED1 immunostaining. In groups II and III, it was observed increased immunoreactivity to GFAP and reexpression of VIM. In group II, ED1-positive cells were noted after the 2 Id day and in group III, after the 3(rd) day. On the 14(th) day post-injection, it was observed a greater quantity of ED1-positive cells in group III than in group II. Apparently, CY did not change the astrocytic response pattern.
Subject: demyelination-remyelination
ethidium bromide
Country: Brasil
Editor: Assoc Arquivos De Neuro- Psiquiatria
Citation: Arquivos De Neuro-psiquiatria. Assoc Arquivos De Neuro- Psiquiatria, v. 64, n. 3B, n. 787, n. 793, 2006.
Rights: aberto
Identifier DOI: 10.1590/S0004-282X2006000500016
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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