Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69949
Type: Artigo de periódico
Title: Immunohistochemical and biochemical assay of MMP-3 in human dentine
Author: Mazzoni, A
Papa, V
Nato, F
Carrilho, M
Tjaderhane, L
Ruggeri, A
Gobbi, P
Mazzotti, G
Tay, FR
Pashley, DH
Breschi, L
Abstract: Objective: The function of endogenous MMP-3 and its distribution within the human dentine is unclear. Thus, the aim of the present study was to assay the presence and distribution of MMP-3 within human sound dentine by means of biochemical and immunohistochemical assays. Methods: Powdered dentine from extracted human teeth was prepared and (1) partially demineralised with 1% H(3)PO(4) for 10 min or (2) untreated (control). The presence of MMP-3 was measured using a colorimetric assay system (QuantiSir (TM), Epigentek, USA). Additional cryo-fractured dentine fragments were processed for immunohistochemical identification of MMP-3 under FEI-SEM. Casein-zymography was used to investigate MMP-3 activity. Results: MMP-3 detected level was 2.732 ng/mu L in partially demineralised dentine powder, whilst it increased to 3.280 ng/mu L in mineralised dentine. The FEI-SEM analysis revealed positive immunolabelling patterns for MMP-3, predominantly localized on the intertubular collagen fibrillar network showing MMP-3 directly or indirectly bound to the collagen fibrils. Casein-zymograms showed positive proteolytic activity for MMP-3 in demineralised dentine powder. Conclusion: The results of the study clearly revealed the presence and distribution of MMP3 in human sound dentine. Whilst the presence was verified, its role is still unclear. Future studies are needed to investigate the possible involvement of MMP-3 in physiological and pathological condition of the dentine-pulp complex. (C) 2011 Elsevier Ltd. All rights reserved.
Subject: MMP-3
Dentine
Dentine bonding agent
Biochemical assay
Immunohistochemistry
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/j.jdent.2011.01.001
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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