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Type: Artigo de periódico
Title: Immunocytochemical localization of cytokines and inducible nitric oxide synthase (NOS) in oral mucosa and lymph nodes of patients with paracoccidioidomycosis
Author: Neworal, EPM
Altemani, A
Mamoni, RL
Noronha, IL
Blotta, MHSL
Abstract: Paracoccidioidomycosis (PCM) is a deep mycosis caused by Paracoccidioides brasiliensis, with high incidence in Brazil. In order to examine the immune response in lesional tissue from patients with PCM, we analyzed cytokines as well as the phenotype of the cell infiltrate. Paraffin-embedded tissue from the oral mucosa of eight patients with the localized adult form (AF) of PCM and from the lymph nodes of 10 patients with the juvenile form (JF) of PCM was analyzed by immunohistochemistry to detect tumor necrosis factor-alpha (TNF-alpha), inducible nitric oxide synthase (NOS), transforming growth factor-beta (TGF-beta) and interleukin-10 (IL-10). Most of the inflammatory cells in the lymph nodes were CD68+ (macrophages, epithelioid and giant cells), while a mixed infiltrate with macrophages, plasma cells and neutrophils was detected in the oral mucosa. TNF-a as well as NOS expression was similar in lymph nodes and oral mucosa, whereas TGF-beta and IL-10 were observed in a larger number of macrophages, epithelioid and giant cells in the lymph nodes, where numerous yeast cells were visualized. The higher expression of anti-inflammatory cytokines (IL-10 and TGF-beta) in lesions of patients with the JF of PCM (lymph nodes) may represent a mechanism by which the fungus evades the host immune response, contributing to a more severe and disseminated form of the disease. (C) 2003 Elsevier Science Ltd. All rights reserved.
Subject: interleukin-10
inducible nitric oxide synthase
transforming growth factor-beta
tumor necrosis factor-alpha
Country: Inglaterra
Editor: Academic Press Ltd Elsevier Science Ltd
Citation: Cytokine. Academic Press Ltd Elsevier Science Ltd, v. 21, n. 5, n. 234, n. 241, 2003.
Rights: fechado
Identifier DOI: 10.1016/S1043-4666(03)00051-6
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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