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Type: Artigo de periódico
Title: Immobilization of horseradish peroxidase onto polyaniline polymers
Author: Fernandes, KF
Lima, CS
Pinho, H
Collins, CH
Abstract: Some chemically synthesized conductive polymers, based on polyaniline (PANI), were used as supports for Horseradish Peroxidase (HRP) immobilization. The polymers were first activated with glutaraldehyde (PANIG) and then HRP was successfully immobilized. Among the tested polymers, those with intermediate oxidation state (PANIG(1), PANIG(2) and PANIG(3)) were more suitable for HRP immobilization than that predominately reduced (PANIG(2)) or oxidized (PANIG(5)). PANIG(1) presented the best performance for HRP immobilization, and the analysis of the protein versus active enzyme retention showed that immobilized HRP maintained 100% native activity. The effects of enzyme concentration, reaction time and pH on the immobilization efficiency were investigated. (C) 2003 Elsevier Science Ltd. All rights reserved.
Subject: polyaniline
horseradish peroxidase
retention efficiency
Country: Inglaterra
Editor: Elsevier Sci Ltd
Citation: Process Biochemistry. Elsevier Sci Ltd, v. 38, n. 9, n. 1379, n. 1384, 2003.
Rights: fechado
Identifier DOI: 10.1016/S0032-9592(03)00021-9
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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