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|Type:||Artigo de periódico|
|Title:||Increased circulating cell-free hemoglobin levels reduce nitric oxide bioavailability in preeclampsia|
|Author:||SANDRIM, Valeria C.|
MONTENEGRO, Marcelo F.
PALEI, Ana C. T.
METZGER, Ingrid F.
SERTORIO, Jonas T. C.
CAVALLI, Ricardo C.
TANUS-SANTOS, Jose E.
|Abstract:||Contrasting with increased nitric oxide (NO) formation during healthy pregnancy, reduced NO bioavailability plays a role in preeclampsia. However, no study has examined whether increased NO consumption by enhanced circulating levels of cell-free hemoglobin plays a role in preeclampsia. We studied 82 pregnant women (38 healthy pregnant and 44 with preeclampsia). To assess NO bioavailability, we measured plasma and whole blood nitrite concentrations using an ozone-based chemiluminescence assay. Plasma ceruloplasmin concentrations and plasma NO consumption (pNOc) were assessed and plasma hemoglobin (pHb) concentrations were measured with a commercial immunoassay. We found lower whole blood and plasma nitrite concentrations in preeclamptic patients (-48 and -39%, respectively; both P<0.05) compared with healthy pregnant women. Plasma samples from preeclamptic women consumed 63% more NO (P=0.003) and had 53% higher pHb and 10% higher ceruloplasmin levels than those found in healthy pregnant women (P<0.01). We found significant positive correlations between pHb and pNOc (r=0.61; P<0.0001), negative correlations between pNOc and whole blood or plasma nitrite concentrations (P=0.02; r=-0.32 and P=0.01: r=-0.34, respectively), and negative correlations between pHb and whole blood or plasma nitrite concentrations (P=0.03; r=-0.36 and P=0.01: r=-0.38, respectively). These findings suggest that increased pHb levels lead to increased NO consumption and lower NO bioavailability in preeclamptic compared with healthy pregnant women. (C) 2010 Elsevier Inc. All rights reserved.|
|Editor:||ELSEVIER SCIENCE INC|
|Citation:||FREE RADICAL BIOLOGY AND MEDICINE, v.49, n.3, p.493-500, 2010|
|Appears in Collections:||FCM - Artigos e Outros Documentos|
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