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Type: Artigo de periódico
Title: On-line molecularly imprinted solid-phase extraction for the selective spectrophotometric determination of nicotine in the urine of smokers
Author: Figueiredo, EC
de Oliveira, DM
de Siqueira, MEPB
Arruda, MAZ
Abstract: This work describes an on-line molecularly imprinted solid-phase extraction (MISPE) method for spectrophotometric determination of nicotine in urine samples of smokers. This method is based on manganese (VII) to manganese (VI) reduction in an alkaline medium, promoted by nicotine. Two wash solutions (1:4 (v/v) acetonitrile:sodium hydroxide - pH 11.4, and nitric acid - pH 2.5) were employed to circumvent interferences. Aqueous solutions containing nicotine plus different possible concomitants (cotinine, anabasine, norcotinine and caffeine) were tested individually. The analytical calibration curve was prepared in urine samples collected from non-smokers and spiked with nicotine standard from 1.1 to 60 mu mol L(-1) (r(2)>0.998). The limit of quantification and the analytical frequency were 1.1 mu mol L(-1) and 11 h(-1). respectively. The precision. evaluated using 3. 10 and 30 mu mol L(-1) nicotine in urine, was 10, 10 and 4% (intra-day precision) and 12, 13 and 5% (inter-day precision), respectively. Accuracy was checked through high performance liquid chromatography and the results did not present significant differences at the 95% confidence level according to the Student's t-test. (c) 2009 Elsevier B.V. All rights reserved
Subject: Molecularly imprinted polymers
Solid-phase extraction
Country: Holanda
Editor: Elsevier Science Bv
Citation: Analytica Chimica Acta. Elsevier Science Bv, v. 635, n. 1, n. 102, n. 107, 2009.
Rights: fechado
Identifier DOI: 10.1016/j.aca.2008.12.045
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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