Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69424
Type: Artigo de periódico
Title: In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus
Author: Anhe, GF
Caperuto, LC
Pereira-Da-Silva, M
Souza, LC
Hirata, AE
Velloso, LA
Cipolla-Neto, J
Carvalho, CRO
Abstract: Melatonin is the pineal hormone that acts via a pertussis toxin-sensitive G-protein to inhibit adenylate cyclase. However, the intracellular signalling effects of melatonin are not completely understood. Melatonin receptors are mainly present in the suprachiasmatic nucleus (SCN) and pars tuberalis of both humans and rats. The SCN directly controls, amongst other mechanisms, the circadian rhythm of plasma glucose concentration. In this study, using immunoprecipitation and immunoblotting, we show that melatonin induces rapid tyrosine phosphorylation and activation of the insulin receptor beta-subunit tyrosine kinase (IR) in the rat hypothalamic suprachiasmatic region. Upon IR activation, tyrosine phosphorylation of IRS-1 was detected. In addition, melatonin induced IRS-1/PI(3)-kinase and IRS-1/SHP-2 associations and downstream AKT serine phosphorylation and MAPK (mitogen-activated protein kinase) phosphorylation, respectively. These results not only indicate a new signal transduction pathway for melatonin, but also a potential cross-talk between melatonin and insulin.
Subject: AKT
GPCR
hypothalamus
insulin receptor
melatonin
tyrosine phosphorylation
Country: Inglaterra
Editor: Blackwell Publishing Ltd
Rights: fechado
Identifier DOI: 10.1111/j.1471-4159.2004.02514.x
Date Issue: 2004
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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