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Type: Artigo de periódico
Title: In vitro assessment of the immediate and prolonged antimicrobial action of chlorhexidine gel as an endodontic irrigant against Enterococcus faecalis
Author: Dametto, FR
Ferraz, CCR
Paula, B
Gomes, FD
Zaia, AA
Teixeira, FB
de Souza, FJ
Abstract: Objective. The aim of this study was to assess in vitro the antimicrobial activity of 2% chlorhexidine gel against Enterococcus faecalis, comparing it to other endodontic irrigants (2% chlorhexidine liquid and 5.25% sodium hypochlorite). Study design. Eighty roots of human mandibular premolars were prepared by serial instrumentation technique, autoclaved, and contaminated for 7 days with E faecalis monocultures. The roots were then divided into 5 groups according to the irrigant substance used during the standardized biomechanical preparation. In order to evaluate the antimicrobial action of the irrigant substances, 3 microbial samples were taken: initial (before the biomechanical preparation); post-treatment (immediately after the biomechanical preparation), and final (7 days after the biomechanical preparation). The microbiological samples were plated to count the colony-forming units (CFU). Results. The 2% chlorhexidine gel and 2% chlorhexidine liquid significantly reduced the E faecalis CFU in the post-treatment and final microbiological samples. The 5.25% sodium hypochlorite also reduced the E faecalis CFU immediately after the root canal instrumentation, but it was not able to keep the root canal free of detectable E faecalis in the final sample. Conclusions. The 2% chlorhexidine gluconate (gel and liquid) antimicrobial ability was more effective than 5.25% sodium hypochlorite in keeping low CFU of E faecalis for 7 days after the biomechanical preparation.
Country: EUA
Editor: Mosby, Inc
Rights: fechado
Identifier DOI: 10.1016/j.tripleo.2004.08.026
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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