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Type: Artigo de periódico
Title: In vitro and in vivo antitumor activity of crude extracts obtained from Brazilian Chromobacterium sp isolates
Author: Menezes, CBA
Silva, BP
Sousa, IMO
Ruiz, ALTG
Spindola, HM
Cabral, E
Eberlin, MN
Tinti, SV
Carvalho, JE
Foglio, MA
Fantinatti-Garboggini, F
Abstract: Natural products produced by microorganisms have been an important source of new substances and lead compounds for the pharmaceutical industry. Chromobacterium violaceum is a Gram-negative beta-proteobacterium, abundant in water and soil in tropical and subtropical regions and it produces violacein, a pigment that has shown great pharmaceutical potential. Crude extracts of five Brazilian isolates of Chromobacterium sp (0.25, 2.5, 25, and 250 mu g/mL) were evaluated in an in vitro antitumor activity assay with nine human tumor cells. Secondary metabolic profiles were analyzed by liquid chromatography and electrospray ionization mass spectrometry resulting in the identification of violacein in all extracts, whereas FK228 was detected only in EtCE 308 and EtCE 592 extracts. AcCE and EtCE 310 extracts showed selectivity for NCI/ADR-RES cells in the in vitro assay and were evaluated in vivo in the solid Ehrlich tumor model, resulting in 50.3 and 54.6% growth inhibition, respectively. The crude extracts of Chromobacterium sp isolates showed potential and selective antitumor activities for certain human tumor cells, making them a potential source of lead compounds. Furthermore, the results suggest that other compounds, in addition to violacein, deoxyviolacein and FK228, may be involved in the antitumor effect observed.
Subject: Chromobacterium sp
Antitumor activity
Secondary metabolites
Genetic diversity
Country: Brasil
Editor: Assoc Bras Divulg Cientifica
Citation: Brazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 46, n. 1, n. 65, n. 70, 2013.
Rights: aberto
Identifier DOI: 10.1590/S0100-879X2012007500167
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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