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Type: Artigo de periódico
Title: In situ activated 3,5-dinitrobenzoic acid covalent attached to nanostructured platform for NADH electrooxidation
Author: Santhiago, M
Lima, PR
Santos, WDR
de Oliveira, AB
Kubota, LT
Abstract: The activation of 3,5-dinitrobenzoic acid (3,5-DNBA) in situ is proposed for electrocatalytic detection of NADH at low overpotentials. Thus, 3,5-DNBA was strategically connected to a nanostructured surface based on MWCNTs and PEI (an amine rich polymer). Then, R-NO/R-NHOH was electrogenerated in situ by cycling the potential between 0.15 and -0.55 V versus SCE. The formation of an intermediate charge transfer complex is proposed for the reaction between NADH and redox mediator. The apparent Michaelis-Menten constant (K(m)) obtained by RDE voltammetry and amperometry was in good agreement, around 10(-5) mol L(-1). The k(obs) for the catalytic reaction evaluated by chronoamperometry and RDE voltammetry, was in good agreement and was found to be around 10(4) L mol(-1) s(-1). The sensor provides a linear response range for NADH from 4.0 up to 42.0 mu mol L(-1) with a sensitivity of 71 nA L mu mol(-1), detection and quantification limits of 1.2 and 4.0 mu mol L(-1) respectively, with a response time of 0.16s. (C) 2009 Elsevier Ltd. All rights reserved.
Subject: 3,5-Dinitrobenzoic acid
Mechanism and kinetics
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.electacta.2009.06.032
Date Issue: 2009
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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