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Type: Artigo de periódico
Title: In search of a tolerance-induction strategy for cow's milk allergies: significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization
Author: Olivier, CE
Lima, RPD
Pinto, DG
dos Santos, RAPG
da Silva, GKM
Lorena, SLS
Villas-Boas, MB
Netto, FM
Zollner, RD
Abstract: OBJECTIVE: To explore the use of beta-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS: Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick test) with native and polymerized beta-lactoglobulin in adult patients with an IgE-mediated allergy to beta-lactoglobulin. In vitro humoral immunoreactivity was analyzed using immunoblotting. Cell-mediated immunoreactivity was analyzed using ex vivo challenges with native and polymerized beta-lactoglobulin and monitored by leukocyte adherence inhibition tests. RESULTS: The skin tests demonstrated that there was a significant reduction in immediate cutaneous reactivity after polymerization. Polymerization did not decrease the immunoblotting detection of s-IgE specific to beta-lactoglobulin. Cell-mediated immunoreactivity, as assessed by ex vivo challenges and leukocyte adherence inhibition tests, did not exhibit significant differences between leukocytes challenged with native versus polymerized beta-lactoglobulin. CONCLUSIONS: The polymerization of beta-lactoglobulin decreased in vivo allergenicity and did not decrease in vitro humoral or ex vivo cell-mediated immunoreactivity. Therefore, we conclude that inducing polymerization using transglutaminase represents a promising technique to produce suitable molecules for the purpose of designing oral/sublingual tolerance induction protocols for the treatment of allergies.
Subject: Beta-lactoglobulin
Milk Hypersensitivity
Leukocyte Adherence Inhibition Test
Immune Tolerancez
Country: Brasil
Editor: Hospital Clinicas, Univ Sao Paulo
Citation: Clinics. Hospital Clinicas, Univ Sao Paulo, v. 67, n. 10, n. 1171, n. 1179, 2012.
Rights: aberto
Identifier DOI: 10.6061/clinics/2012(10)09
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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