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|Type:||Artigo de periódico|
|Title:||Improving metallomics information related to transgenic and non-transgenic soybean seeds using 2D-HPLC-ICP-MS and ESI-MS/MS|
|Abstract:||This work reports the use of 2D-HPLC-ICP-MS to enlarge metallomics information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed the identification of three metal fractions: the first corresponding to molecular weights from 38.1 to 181.1 kDa, the second from 8.2 to 17.2 kDa and the third from 0.4 to 3.8 kDa. In a second dimension, using anion exchange chromatography (AEX), three sub-fractions containing Fe, Mg and Mn, one containing Cu, and three containing Co, Cu, Mg, Mn and Zn were obtained. After these separations, 33 proteins were identified using the ESI-MS/MS technique, and divided into four functional categories: plant growth/cell division, protein destination and storage, metabolism and unclassified proteins. Among the identified proteins, proteins previously related to metals were found.|
|Editor:||Royal Soc Chemistry|
|Appears in Collections:||Artigos e Materiais de Revistas Científicas - Unicamp|
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