Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69232
Type: Artigo de periódico
Title: IMAGE-ANALYSIS - VIDEO SYSTEM ADEQUACY FOR THE ASSORTMENT OF NUCLEAR PHENOTYPES BASED ON CHROMATIN TEXTURE EVALUATION
Author: MELLO, MLS
VIDAL, BD
PLANDING, W
SCHENCK, U
Abstract: The discrimination of nuclear phenotypes of Feulgen-stained ras-transformed and nontransformed NIH 3T3 cells previously determined by image analysis using the scanning microspectrophotometry system, was investigated with the video system. A reasonable matching of the Feulgen-DNA values obtain ed with the two methods was demonstrated. Chromatin texture evaluated on the basis of the scatter diagram relating condensed chromatin relative areas vs. an average absorption ratio could also be assessed with the video system. The video image analysis system was effective in distinguishing nuclear phenotypes which differ in chromatin supraorganization in ras-transformed as well as in nontransformed NIH 3T3 cells, in distinguishing phenotypes not visually discernible from each other (transformed vs. nontransformed cells) and in discriminating among the cell lines used.
Country: Japão
Editor: Japan Soc Histochemistry & Cytochemistry
Rights: aberto
Date Issue: 1994
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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