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Type: Artigo de periódico
Title: Identification of selenium in the leaf protein of sunflowers by a combination of 2D-PAGE and laser ablation ICP-MS
Author: da Silva, MAO
Arruda, MAZ
Abstract: We report on a method for the identification of selenium-containing proteins in an extract of sunflower leafs. It is based on the separation of the proteins by 2-dimensional gel electrophoresis, followed by detection of selenium via laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The laser system was operated in a raster mode at 100 mu m s(-1) and proved to be an efficient alternative in the search for selenoproteins in the spots of the gels. The instrumental parameters were optimized in terms of plasma energy and application of optimal reaction cell conditions, and the detection of the mass (SeO+)-Se-80-O-16 which enabled the elimination of interfering species. Selenium was identified in 9.6% of the analyzed spots, indicating its random incorporation into the primary structure of the proteins.
Subject: LA-ICP-MS
Gel electrophoresis
Country: Austria
Editor: Springer Wien
Rights: fechado
Identifier DOI: 10.1007/s00604-011-0700-6
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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