Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69167
Type: Artigo de periódico
Title: Identification of nuclear factor-kappa B sites in the Slc2a4 gene promoter
Author: Furuya, DT
Neri, EA
Poletto, AC
Anhe, GF
Freitas, HS
Campello, RS
Reboucas, NA
Machado, UF
Abstract: Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-kappa B transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative kappa B sites located inside -83/-62 and -134/-113 bp. Eletrophoretic mobility assay showed that p50 and p65 NF-kappa B subunits bind to both putative kappa B sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-kappa B binds to the -134/-113 bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. The current findings demonstrate the existence of two kappa B sites in Slc2a4 gene promote, and that NF-kappa B has a direct repressor effect upon the Slc2a4 gene, providing an important link between insulin resistance and inflammation. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
Subject: NF-kappa B
Slc2a4
GLUT4
Insulin resistance
Inflammation
Gene reporter
Country: Irlanda
Editor: Elsevier Ireland Ltd
Rights: fechado
Identifier DOI: 10.1016/j.mce.2013.01.019
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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