Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69160
Type: Artigo de periódico
Title: IDENTIFICATION OF MICROCISTIN LR AT THE MOLECULAR LEVEL USING ATOMIC FORCE MICROSCOPY
Author: Etchegaray, A
Bueno, CD
Teschke, O
Abstract: IDENTIFICATION OF MICROCISTIN LR AT THE MOLECULAR LEVEL USING ATOMIC FORCE MICROSCOPY. Microcystins are non-ribosomal peptides that must he detected for its health concern. Here, microcystin LR and its specific antibody were respectively tethered to the substrate and to the tip of an atomic force microscope, after surface functionalization using 3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmed comparing topographic images taken on bare and modified tips. Force versus distance curves were successfully used to measure the specific antibody-antigen interactions comparing with a control in which microcystin was initially blocked by incubation with free antibodies. The results showed unequivocally the specific recognition of MLR, suggesting that this method could be useful for biosensor development.
Subject: microcystin LR
atomic force microscope
biosensor development
Country: Brasil
Editor: Soc Brasileira Quimica
Rights: aberto
Identifier DOI: 10.1590/S0100-40422010000900004
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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