Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/69150
Type: Artigo de periódico
Title: Identification of four Eucalyptus genes potentially involved in cell wall biosynthesis and evolutionarily related to SHINE transcription factors
Author: Marques, WL
Salazar, MM
Camargo, ELO
Lepikson-Neto, J
Tiburcio, RA
do Nascimento, LC
Pereira, GAG
Abstract: Recently, a new Arabidopsis thaliana master regulator of plant cell wall biosynthesis was characterized. It was named SHINE transcription factor (SHINE TF). This work searched for homologous genes in Eucalyptus grandis genome draft. RNAseq data, phylogeny analysis and qRT-PCR experiments were performed to complement SHINE gene analysis. By similarity searches using A. thaliana SHINE genes, four sequences were identified in Eucalyptus. Two of them contain all conserved motifs and characteristic features of this family, being assumed as true SHINE TFs and named EgrSHN1 and EgrSHN2. The other two sequences contain an incomplete 'mm' motif and were not considered true SHINE TFs, being further referred as Egr33m and Egr40m. Expression analysis revealed that EgrSHN1 is more expressed in flowers than in leaves and immature xylem, and both EgrSHN1 and EgrSHN2 are absent from adult xylem RNAseq libraries. This expression profile is similar to A. thaliana orthologues. On the other hand, Egr33m and Egr40m expression was detected in adult xylems. The phylogenetic studies indicate that both EgrSHNs were originated by gene duplication events which, together with gene loss, are hypothesized as common events in SHINE evolution. In conclusion, it is possible that the overexpression of SHINE genes in Eucalyptus xylem can generate information about wood formation processes, allowing an effective increase in forest plantation productivity.
Subject: Eucalyptus
SHINE transcription factors
Secondary cell wall
Plant cell wall regulation
Country: Holanda
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s10725-012-9754-7
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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